Biomarcadores de estresse oxidativo e do metabolismo muscular na saliva de equinos

Abstract

Saliva has the potential to be used as a sample capable of providing biomarkers of oxidative, metabolic and electrolytic stress for animals in athletic activity, and it can be drawn non-invasively, reducing the discomfort of the animals. The objective of this study was to evaluate the potential of saliva as a biological sample for sampling and determining biomarkers of oxidative stress (malondealdehyde and total antioxidant capacity) and metabolism (for creatine kinase, aspartate aminotransferase, lactate dehydrogenase, lactate, total protein and uric acid). Mangalarga Marchador horses aged between 3 and 15 years old were used from the Federal Rural University of Rio de Janeiro´s farm. Saliva was draw after cleaning up the oral cavity, after that a hydrophilic cotton roller helded by a long hemostatic tweezers was inserted in the mouth of the horses, and blood samples were obtained by venipuncture of the jugular vein immediately after cleaning the mouth. The mean of salivary results were: total protein 0.9 g/dL, uric acid 1.2 mg/dL, aspartate aminotransferase 528 IUI/L, creatine kinase 72.1 IUI/L, lactate dehydrogenase 2268.2 IUI/L, lactate 3.9 mmol/L and the median of malondialdehyde was 1.6 nmol/ml. It is concluded that salivary enzyme values collected have low or zero correlation with serum values when harvested simultaneously, so the use of saliva as a biomarker depends on the development of specific researches and saliva can be used instead of plasma or serum for analysis of TBARS