Obtenção de compostos de alto valor comercial a partir de biomassa microalgal por extração com fluido supercrítico (EFS)

Abstract

This study evaluated the potential of the extraction technique with supercritical fluid for obtaining high commercial value compounds (carotenoids and chlorophyll-a) biomass of Spirulina spp., And the performance of this microalgae in low cost culture medium, aiming at high productivity in biomass and pigments. The supercritical fluid extraction showed high selectivity in relation to the achievement of pigments, particularly the carotenoids. The larger supercritical extraction yields for both pigments were observed at 200bar under isothermal condition of 40 ° C, the concentrations being 13.55 mg L-1 of chlorophyll-a and 7.82 mg L-1 of total carotenoid the methanol extract used the extracted material. Chlorophyll extraction with respect to carotenoids increased with the increase in pressure, indicating the possibility of an extraction process in two steps. On crops, it was observed a great performance from the microalgae cultivated in the Mod.III (2.5) medium, compared to cultures performed in the Zarrouk medium, where it reached, after eight days, a concentration in the final biomass of 1,28 g L-1, chlorophyll concentration of 11,51 μg mL-1 and carotenoids 3,98 μg mL-1.