Aspectos histológicos, histoquímicos e imuno-histoquímicos do intestino e pâncreas de morcegos da família Phyllostomidae (Mammalia, Chiroptera)

Abstract

Histological, histochemical and immunohistochemical studies have sought to obtain data on the gastrointestinal tube (GIT) of bats, due to the fact that these animals have a diversified diet. The present study aimed to describe the histological and histochemical characteristics of the intestine and pancreas of four species of bat the family Phyllostomidae (Dryadonycteris, Capixaba, Pygoderma, bilabiatum, Chiroderma, Villosum and Phyllostomus discolor), as well as to analyze the presence of endocrine cells in these organs of the species studied, seeking to relate interspecifically the characteristics found. The animals were analyzed transferred from the zoological collection of the Microbiology Laboratory of the Department of Animal Biology (DBA) of the Universidade Federal Rural do Rio de Janeiro (UFRRJ).The organs were fixed (10% formalin) and conditioned in 70% alcohol. Subsequently, it was forwarded to the Laboratory of Histology and Embryology of UFRRJ, in which they were submitted to routine histological processing and immunohistochemical tests. Histologically the intestine and the pancreas of the species studied were similar. As for the immunohistochemical tests, two types of immunoreactive cells (IR) were identified in the intestinal mucosa of the studied species, 5HT and CCK producing cells. In the pancreas, immunoreactive cells (IR) were seen on insulin, glucagon on somatostatin and cholecystokinin. Through this study, there were differences in the distribution and relative frequency of endocrine cells in the small intestine of the animals studied. While in the pancreas species similarity was observed when compared to the histology and distribution of endocrine cells studied.